E-GEOD-8740 - Transcription profiling of Arabidopsis after 5 min mechanical wounding

Status
Submitted on 9 August 2007, released on 16 June 2008, last updated on 2 May 2014
Organism
Arabidopsis thaliana
Samples (24)
Array (1)
Protocols (6)
Description
Plants are continuously exposed to a myriad of abiotic and biotic stresses. However, the molecular mechanisms by which these stress signals are perceived and transduced are poorly understood. To begin to identify primary stress signal transduction components we have focused on genes that respond rapidly (within 5 min) to stress signals. Because it has been hypothesized that detection of physical stress is a mechanism common to mounting a response against a broad range of environmental stresses, we have utilized mechanical wounding as the stress stimulus and performed whole genome microarray analysis of Arabidopsis thaliana leaf tissue. This led to the identification of a number of rapid wound responsive (RWR) genes. Comparison of RWR genes with published abiotic and biotic stress microarray datasets demonstrates a large overlap across a wide range of environmental stresses. Interestingly, RWR genes also exhibit a striking level and pattern of circadian regulation, with induced and repressed genes displaying antiphasic rhythms. Using bioinformatic analysis, we identified a novel motif overrepresented in the promoters of RWR genes, herein designated as the Rapid Stress Response Element (RSRE). We demonstrate in transgenic plants that multimerized RSREs are sufficient to confer a rapid response to both biotic and abiotic stresses in vivo, thereby establishing the functional involvement of this motif in primary transcriptional stress responses. Collectively, our data provide evidence for a novel cis-element that is distributed across the promoters of an array of diverse stress-responsive genes, poised to respond immediately and coordinately to stress signals. This structure suggests that plants may have a transcriptional network resembling the general stress signaling pathway in yeast and that the RSRE element may provide the key to this coordinate regulation. Experiment Overall Design: Three biological replicates of pooled plants were used for each treatment (Not wounded vs 5 min wounded). Each biological replicate is comprised of 2 technical replicates and a dye swap was performed for each technical replicate.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Justin Walley
Citation
Mechanical stress induces biotic and abiotic stress responses via a novel cis-element. Justin W Walley, Sean Coughlan, Matthew E Hudson, Michael F Covington, Roy Kaspi, Gopalan Banu, Stacey L Harmer, Katayoon Dehesh. PLoS Genet 3(10):1800-12 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8740.idf.txt
Sample and data relationshipE-GEOD-8740.sdrf.txt
Processed data (1)E-GEOD-8740.processed.1.zip
Array designA-AGIL-4.adf.txt
Links