E-GEOD-8684 - Transcription profiling of mouse 3T3-L1 adipocyte tissue culture treated with cis-9,trans-11 conjugated linoleic acid(c9t11 CLA)

Status
Submitted on 3 August 2007, released on 16 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (10)
Array (1)
Protocols (4)
Description
Trans-10, Cis-12 conjugated linoleic acid (t10c12 CLA) causes fat loss in mouse 3T3-L1 adipocyte tissue culture; however cis-9, trans-11 CLA does not (this series). The early transcriptome changes were analyzed using high-density microarrays to better characterize the signaling pathways responding to c9t11 CLA. Their gene expression responses between 8 to 12 hr after treatment showed no gene expression changes indicative of an integrated stress response (ISR). Experiment Overall Design: Mouse 3T3-L1 RNA for each time point was isolated from control and treatment samples for analysis on microarrays; there are four biological reps for the controls for each timepoint, and only one rep for the treatment.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Trans-10, cis-12 conjugated linoleic acid activates the integrated stress response pathway in adipocytes. P Christopher LaRosa, Jean-Jack M Riethoven, Han Chen, Yuannan Xia, You Zhou, Mei Chen, Jess Miner, Michael E Fromm. Physiol Genomics 31(3):544-53 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8684.idf.txt
Sample and data relationshipE-GEOD-8684.sdrf.txt
Raw data (1)E-GEOD-8684.raw.1.zip
Processed data (1)E-GEOD-8684.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-8684.eSet.r
Links