E-GEOD-8683 - Transcription profiling of mouse 3T3-L1 tissue (preadipocytes) treated with trans-10,Cis-12 conjugated linoleic acid(t10c12 CLA)

Status
Submitted on 3 August 2007, released on 16 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (11)
Array (1)
Protocols (4)
Description
Trans-10, Cis-12 conjugated linoleic acid (t10c12 CLA) causes fat loss in mouse white adipose tissue (WAT) and 3T3-L1 adipocyte tissue culture; however in preadipocyte tissue (this series) the UPS/ISR and fat loss is not detected. The early transcriptome changes in 3T3-L1 preadipocyte tissue culture were analyzed using high-density microarrays to better characterize the signaling pathways responding to t10c12 CLA. Their gene expression responses between 4 to 12 hr after treatment do not show a set of genes indicative of an integrated stress response (ISR). Experiment Overall Design: Mouse 3T3-L1 RNA for each time point was isolated from two control (LA) and two treatment (CLA) samples for analysis on four microarrays.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Trans-10, cis-12 conjugated linoleic acid activates the integrated stress response pathway in adipocytes. P Christopher LaRosa, Jean-Jack M Riethoven, Han Chen, Yuannan Xia, You Zhou, Mei Chen, Jess Miner, Michael E Fromm. Physiol Genomics 31(3):544-53 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8683.idf.txt
Sample and data relationshipE-GEOD-8683.sdrf.txt
Raw data (1)E-GEOD-8683.raw.1.zip
Processed data (1)E-GEOD-8683.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-8683.eSet.r
Links