E-GEOD-8683 - Transcription profiling of mouse 3T3-L1 tissue (preadipocytes) treated with trans-10,Cis-12 conjugated linoleic acid(t10c12 CLA)
Submitted on 3 August 2007, released on 16 June 2008, last updated on 27 March 2012
Trans-10, Cis-12 conjugated linoleic acid (t10c12 CLA) causes fat loss in mouse white adipose tissue (WAT) and 3T3-L1 adipocyte tissue culture; however in preadipocyte tissue (this series) the UPS/ISR and fat loss is not detected. The early transcriptome changes in 3T3-L1 preadipocyte tissue culture were analyzed using high-density microarrays to better characterize the signaling pathways responding to t10c12 CLA. Their gene expression responses between 4 to 12 hr after treatment do not show a set of genes indicative of an integrated stress response (ISR). Experiment Overall Design: Mouse 3T3-L1 RNA for each time point was isolated from two control (LA) and two treatment (CLA) samples for analysis on four microarrays.
transcription profiling by array, unknown experiment type
Trans-10, cis-12 conjugated linoleic acid activates the integrated stress response pathway in adipocytes. P Christopher LaRosa, Jean-Jack M Riethoven, Han Chen, Yuannan Xia, You Zhou, Mei Chen, Jess Miner, Michael E Fromm. Physiol Genomics 31(3):544-53 (2007)