E-GEOD-8682 - Transcription profiling of mouse 3T3-L1 adipocyte tissue culture treated with tunicamycin

Status
Submitted on 3 August 2007, released on 16 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (4)
Description
Tunycamcin induces UPR/ISR and Inflammation in mouse 3T3-L1 adipocyte tissue culture. The early transcriptome changes were analyzed using high-density microarrays to better characterize the signaling pathways responding to tunicamycin, to be compared with similar experiments with CLA as the treatment. Their gene expression responses between 4 to 12 hr after treatment showed a common set of early gene expression changes indicative of a UPR/Inflammation stress response. Experiment Overall Design: Mouse 3T3-L1 RNA for each time point was isolated from control and treatment samples for analysis on microarrays with two biological reps.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Trans-10, cis-12 conjugated linoleic acid activates the integrated stress response pathway in adipocytes. P Christopher LaRosa, Jean-Jack M Riethoven, Han Chen, Yuannan Xia, You Zhou, Mei Chen, Jess Miner, Michael E Fromm. Physiol Genomics 31(3):544-53 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8682.idf.txt
Sample and data relationshipE-GEOD-8682.sdrf.txt
Raw data (1)E-GEOD-8682.raw.1.zip
Processed data (1)E-GEOD-8682.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-8682.eSet.r
Links