E-GEOD-868 - Transcription profiling of wildtype or Hyp (X-linked hypophosphatemic) mouse kidney after feeding control or low phosphate diet to study changes in gene expression in response to low-phosphate diet.
Submitted on 10 December 2003, released on 13 December 2007, last updated on 27 March 2012
This is a study of the change in gene expression in mouse kidney after feeding control (1.0% P) or low phosphate diet (0.03% P) for 3 or 5 days to normal or Hyp (X-linked hypophosphatemic) mice at 5 weeks of age. The mice were C57BL/6J. Normal wild-type mice, hemizygous Hyp (Hyp/Y) male mice, or heterozygous (Hyp/+) female mice were used. The gene is dominant, so that the two genders are equally affected with low renal retention of phosphate and severity of their bone disease. RNA from 3 mice were pooled for each microarray. The arrays were processed as described in the Affymetrix GeneChip Expression Analysis Technical Manual (copyright 2001, Affymetrix, Inc., Santa Clara, CA), rev. 1, Part number 701021. The data for each array were scaled to an average signal value of 500 for all genes on the array.
transcription profiling by array, co-expression, growth condition, individual genetic characteristics, sex
The genomic response of the mouse kidney to low-phosphate diet is altered in X-linked hypophosphatemia. Martha H Meyer, Emily Dulde, Ralph A Meyer. Physiol Genomics 18(1):04-Nov (2004)