E-GEOD-8658 - Transcription profiling by array of human monocytes from patients with dominant-negative mutations in PPARgamma, or treated with rosiglitazone

Status
Released on 16 June 2008, last updated on 28 February 2012
Organism
Homo sapiens
Samples (63)
Array (1)
Protocols (20)
Description
In order to gain insights into how PPARg regulates different facets of dendritic cell (DC) differentiation, we sought to identify PPARg regulated genes and gene networks in monocyte-derived dendritic cells using global gene expression profiling. We employed an exogenous ligand activation approach using a selective PPARg ligand (rosiglitazone abbreviated as RSG). In addition, we have defined culture conditions in which human serum (HS) induces PPARg activation via a yet uncharacterized endogenous mechanism. We also compared the gene expression profile of developing dendritic cells obtained from patients harboring dominant negative mutations of the PPARg receptor (C114R and C131Y). Experiment Overall Design: Monocytes were cultured for 6, 24 hours or 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF; cytokine treatment was repeated at day 3. Cells were obtained from 12 healthy individuals (6 biological replicates; the 6 and 24 hours samples were obtained from a single individual but the 5 days samples from a different one). Ligands were added at the beginning of differentiation. The 6 and 24 hours cells were treated with vehicle (DC) or 1 uM rosiglitazone (DC RSG), in the case of 5 day cultured cells 2.5 uM RSG was used. Cells were cultured in RPMI plus 10% FBS, in the case of DC4-DC6 cells were also cultured in human AB serum (DC HS). Finally we also obtained cells from patients harboring point mutations of the PPARg receptor (C114R and C131Y)
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
PPARgamma regulates the function of human dendritic cells primarily by altering lipid metabolism. Istvan Szatmari, Daniel Töröcsik, Maura Agostini, Tibor Nagy, Mark Gurnell, Endre Barta, Krishna Chatterjee, Laszlo Nagy. , Europe PMC 17664351
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8658.idf.txt
Sample and data relationshipE-GEOD-8658.sdrf.txt
Raw data (3)E-GEOD-8658.raw.1.zip, E-GEOD-8658.raw.2.zip, E-GEOD-8658.raw.3.zip
Processed data (1)E-GEOD-8658.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-8658.eSet.r
Links