E-GEOD-85 - Transcription profiling by array of mice mutant for aire

Status
Released on 15 December 2007, last updated on 30 April 2015
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (2)
Description
Mice used were B6/129 F2's, 3-5 weeks of age, either wild type or with both copies of the autoimmune regulator gene (aire, GenBank #AF079536) disrupted. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. RNA was twice-amplified using a T7 polymerase-based method.
Experiment types
transcription profiling by array, co-expression, individual genetic characteristics
Contact
Citation
Projection of an immunological self shadow within the thymus by the aire protein. Mark S Anderson, Emily S Venanzi, Ludger Klein, Zhibin Chen, Stuart P Berzins, Shannon J Turley, Harald von Boehmer, Roderick Bronson, Andrée Dierich, Christophe Benoist, Diane Mathis.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-85.idf.txt
Sample and data relationshipE-GEOD-85.sdrf.txt
Raw data (1)E-GEOD-85.raw.1.zip
Processed data (1)E-GEOD-85.processed.1.zip
Array designA-AFFY-6.adf.txt
Links