E-GEOD-8498 - Transcription profiling of mouse CAD cells transfected with miR-124a-2 reveals the microRNA miR-124 Promotes neuronal differentiation by triggering brain-specific alternative pre-mRNA splicing

Status
Submitted on 16 July 2007, released on 16 June 2008, last updated on 10 June 2011
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
Both microRNAs and alternative pre-mRNA splicing have been implicated in the development of the nervous system (NS), but functional interactions between these two pathways are poorly understood. We demonstrate that the neuron-specific microRNA miR-124a directly targets PTBP1/PTB/hnRNPI mRNA, which encodes a global repressor of alternative pre-mRNA splicing in non-neuronal cells. Among the targets of PTBP1 is a critical cassette exon in the pre-mRNA of PTBP2/nPTB/brPTB, an NS-enriched PTBP1 homolog. When this exon is skipped, PTBP2 mRNA is subject to nonsense-mediated decay. During neuronal differentiation, miR-124a reduces PTBP1 levels leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. These events culminate in the transition from non-NS to NS-specific alternative splicing patterns. We also present evidence that miR-124a plays a key role in the differentiation of progenitor cells to mature neurons. Thus, miR-124a promotes NS development at least in part by regulating an intricate network of NS-specific alternative splicing. We used microarrays to detail the global programme of gene expression of CAD cells over-expressing miR-124a-2. Experiment Overall Design: Expression data from CAD cells transfected with plasmid expressing miR-124a-2.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8498.idf.txt
Sample and data relationshipE-GEOD-8498.sdrf.txt
Raw data (1)E-GEOD-8498.raw.1.zip
Processed data (1)E-GEOD-8498.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-8498.eSet.r
Links