E-GEOD-8444 - Transcription profiling of rat tracheal epithelial RTEC11 cells

Status
Submitted on 11 July 2007, released on 16 June 2008, last updated on 10 June 2011
Organism
Rattus norvegicus
Samples (4)
Array (1)
Protocols (4)
Description
We performed global scale microarray analysis to identify detailed mechanisms by which nonpermissive temperature induces cell growth arrest and differentiation in tracheal epithelial RTEC11 cells harboring temperature-sensitive simian virus 40 large T-antigen by using an Affymetrix GeneChip system. Tracheal epithelial RTEC11 cells used in this study were derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen. Although the cells grew continuously at the permissive temperature, the nonpermissive temperature led to cell growth arrest and differentiation. Experiment Overall Design: A rat conditionally immortalized tracheal epithelial cell line, RTEC11, derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen was used. RTEC11 cells were cultured at permissive (33°C) or nonpermissive (39°C) temperature for 3 days.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8444.idf.txt
Sample and data relationshipE-GEOD-8444.sdrf.txt
Raw data (1)E-GEOD-8444.raw.1.zip
Processed data (1)E-GEOD-8444.processed.1.zip
Array designA-AFFY-25.adf.txt
R ExpressionSetE-GEOD-8444.eSet.r
Links