E-GEOD-8441 - Transcription profiling of human skeletal muscle from 55-80 year old individuals fed low and high protein diets

Status
Submitted on 11 July 2007, released on 16 June 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (22)
Array (1)
Protocols (6)
Description
Inadequate dietary protein intake causes adverse changes in the morphology and function of skeletal muscle. These changes may be reflected in early alterations in muscle mRNA levels. Microarray analysis was used to assess whether inadequate protein intake differentially affects skeletal muscle transcript levels and expression profiles in older adults. Experiment Overall Design: 11 older males and females (aged 55-80 y) consumed controlled diets that contained 1.2 g protein•kg-1•d-1 (adequate protein) for one wk and then 0.5 g protein•kg-1•d-1 (inadequate protein; IP group; n=11) for a second wk. RNA was isolated from fasting-state vastus lateralis biopsies obtained at the end of each period and the transcript levels were determined using the Affymetrix U133A array
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Inadequate protein intake affects skeletal muscle transcript profiles in older humans. Anna E Thalacker-Mercer, James C Fleet, Bruce A Craig, Nadine S Carnell, Wayne W Campbell. Am J Clin Nutr 85(5):1344-52 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8441.idf.txt
Sample and data relationshipE-GEOD-8441.sdrf.txt
Raw data (1)E-GEOD-8441.raw.1.zip
Processed data (1)E-GEOD-8441.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-8441.eSet.r
Links