E-GEOD-8438 - Chromatin immunoprecipitation of human HEK cells immunoprecipitated by Staufen1

Status
Submitted on 11 July 2007, released on 16 June 2008, last updated on 2 May 2014
Organism
Homo sapiens
Samples (7)
Array (1)
Protocols (9)
Description
In human cells, Staufen1 is double-stranded RNA-binding protein involved in several cellular functions including mRNA localization, translation and decay. We used a genome wide approach to identify and compare the mRNA targets of mammalian Staufen1. The mRNA content of Staufen1 mRNPs was identified by probing DNA microarrays with probes derived from mRNAs isolated from immunopurified Staufen-containing complexes following transfection of HEK293T cells with a Stau1-HA expressor. Our results indicate that 7% of the cellular RNAs expressed in HEK293T cells are found in Stau1-containing mRNPs. There is a predominance of mRNAs involved in cell metabolism, transport, transcription, regulation of cell processes and catalytic activity. Experiment Overall Design: HEK293 cells were transiantly transfected with plasmids coding for Staufen1-HA. Cell extracts were immunoprecipitated using anti-HA antibodies and co-immunoprecipitated mRNAs were purified and used to hybridize microarrays. As control, cell extract from mock transfected cells were used.
Experiment types
ChIP-chip by array, unknown experiment type
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8438.idf.txt
Sample and data relationshipE-GEOD-8438.sdrf.txt
Raw data (1)E-GEOD-8438.raw.1.zip
Processed data (1)E-GEOD-8438.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-8438.eSet.r
Links