E-GEOD-8018 - Transcription profiling of quail MBH under short and long day conditions.

Status
Submitted on 5 June 2007, released on 6 November 2008, last updated on 2 May 2014
Organism
Coturnix japonica
Samples (24)
Array (1)
Protocols (7)
Description
Various physiological processes and behaviours are controlled by changing daylength. To dissect genes involved in the photoperiodic changes in physiology and behaviour, global expression analysis was performed using quail kept under short and long day conditions. Experiment Overall Design: The mediobasal hypothalamus (MBH) of quail kept under short day condition (6h light:18h dark) or long day condition (20h light:4h dark) were collected for RNA extraction. Pooled total RNA from three MBHs was labelled and hybridized on Affymetrix microarrays. Samples were collected from 6 birds every 4h during a 24 h cycle and were analyzed in duplicate set of array (two biological replicates).
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Thyrotrophin in the pars tuberalis triggers photoperiodic response. Nobuhiro Nakao, Hiroko Ono, Takashi Yamamura, Tsubasa Anraku, Tsuyoshi Takagi, Kumiko Higashi, Shinobu Yasuo, Yasuhiro Katou, Saburo Kageyama, Yumiko Uno, Takeya Kasukawa, Masayuki Iigo, Peter J Sharp, Atsushi Iwasawa, Yutaka Suzuki, Sumio Sugano, Teruyuki Niimi, Makoto Mizutani, Takao Namikawa, Shizufumi Ebihara, Hiroki R Ueda, Takashi Yoshimura. Nature 452(7185):317-22 (2008)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8018.idf.txt
Sample and data relationshipE-GEOD-8018.sdrf.txt
Raw data (1)E-GEOD-8018.raw.1.zip
Processed data (1)E-GEOD-8018.processed.1.zip
Array designA-AFFY-103.adf.txt
R ExpressionSetE-GEOD-8018.eSet.r
Links