E-GEOD-8017 - Transcription profiling of Coturnix japonica during the photoinduction process.

Status
Submitted on 5 June 2007, released on 6 November 2008, last updated on 2 May 2014
Organism
Coturnix japonica
Samples (42)
Array (1)
Protocols (7)
Description
The molecular mechanism of photoperiodic time measurement remains unknown in animals. Japanese quail is an excellent model for studying these phenomena because of their rapid and dramatic response to photoperiod. When quail are transferred from short to long day conditions, increase in plasma gonadotrophin (luteinising hormone: LH) can be observed by the end of the first long day and this phenomenon is called “first day release model”. To dissect the system dynamics and network structure regulating photoperiodism, we examined global gene expression using high-density oligonucleotide microarray for chicken (Affymetrix Chicken Genome Array). Experiment Overall Design: The mediobasal hypothalamus (MBH) of quail was collected from 6 birds for each time point during the photoinduction process. Pooled total RNA from three MBHs was labelled and hybridized to each Affymetrix chicken genome array. Samples were analyzed in duplicate set of array (two biological replicate).
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Thyrotrophin in the pars tuberalis triggers photoperiodic response. Nobuhiro Nakao, Hiroko Ono, Takashi Yamamura, Tsubasa Anraku, Tsuyoshi Takagi, Kumiko Higashi, Shinobu Yasuo, Yasuhiro Katou, Saburo Kageyama, Yumiko Uno, Takeya Kasukawa, Masayuki Iigo, Peter J Sharp, Atsushi Iwasawa, Yutaka Suzuki, Sumio Sugano, Teruyuki Niimi, Makoto Mizutani, Takao Namikawa, Shizufumi Ebihara, Hiroki R Ueda, Takashi Yoshimura. Nature 452(7185):317-22 (2008)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-8017.idf.txt
Sample and data relationshipE-GEOD-8017.sdrf.txt
Raw data (1)E-GEOD-8017.raw.1.zip
Processed data (1)E-GEOD-8017.processed.1.zip
Array designA-AFFY-103.adf.txt
R ExpressionSetE-GEOD-8017.eSet.r
Links