E-GEOD-7757 - Transcription profiling of leukemia samples prepared using different RNA isolation procedures
Submitted on 8 May 2007, released on 6 November 2008, last updated on 27 March 2012
Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. For the first time, we present a study focused on analyzing the impact of different RNA preparation procedures on gene expression data for different subtypes of pediatric acute leukemias. Experiment Overall Design: We systematically assessed the impact of three different total RNA isolation procedures on variation in expression data: method A: lysis of mononuclear cells, followed by lysate homogenization and RNA extraction; method B: organic solvent based RNA isolation, and method C: organic solvent based RNA isolation followed by purification.
transcription profiling by array, unknown experiment type
New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures. Marta Campo Dell'Orto, Andrea Zangrando, Luca Trentin, Rui Li, Wei-min Liu, Geertruy te Kronnie, Giuseppe Basso, Alexander Kohlmann.