E-GEOD-7757 - Transcription profiling of leukemia samples prepared using different RNA isolation procedures

Status
Submitted on 8 May 2007, released on 6 November 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (99)
Array (1)
Protocols (3)
Description
Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. For the first time, we present a study focused on analyzing the impact of different RNA preparation procedures on gene expression data for different subtypes of pediatric acute leukemias. Experiment Overall Design: We systematically assessed the impact of three different total RNA isolation procedures on variation in expression data: method A: lysis of mononuclear cells, followed by lysate homogenization and RNA extraction; method B: organic solvent based RNA isolation, and method C: organic solvent based RNA isolation followed by purification.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures. Marta Campo Dell'Orto, Andrea Zangrando, Luca Trentin, Rui Li, Wei-min Liu, Geertruy te Kronnie, Giuseppe Basso, Alexander Kohlmann.
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