E-GEOD-7430 - Transcription profiling of mouse pancreas at embryonic days 14.5 and 16.5 from animals lacking beta-catenin, a mediator of Wnt signaling and a component of the cadherin-catenin epithelial adhesion complex

Status
Submitted on 2 April 2007, released on 13 December 2007, last updated on 27 March 2012
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (4)
Description
Beta-catenin is an essential mediator of canonical Wnt signaling and a central component of the cadherin-catenin epithelial adhesion complex. Dysregulation of beta-catenin expression has been described in pancreatic neoplasia. Newly published studies have suggested that beta-catenin is critical for normal pancreatic development although these reports reached somewhat different conclusions. In addition, the molecular mechanisms by which loss of beta-catenin affects pancreas development are not well understood. The goals of this study then were; 1] to further investigate the role of beta-catenin in pancreatic development using a conditional knockout approach and 2] to identify possible mechanisms by which loss of beta-catenin disrupts pancreatic development. A Pdx1-cre mouse line was used to delete a floxed beta-catenin allele specifically in the developing pancreas, and embryonic pancreata were studied by immunohistochemistry and microarray analysis. Experiment Overall Design: Parameter: embryonic pancreas from wild type and Pdx1Cre/+; beta-cateninflox/flox Experiment Overall Design: Sample type: RNA Experiment Overall Design: Source name: Embryonic day 14.6 and 16.5 pancreas Experiment Overall Design: Organism: Mus Musculus Experiment Overall Design: Strain: Derived from an intercross of Pdx1Cre/+ ; beta-cateninflox/+ mice. Experiment Overall Design: Extracted molecule:Total RNA Experiment Overall Design: In the study, we hybridized RNA from Embryonic day 14.6 and 16.5 pancreas of wild type (WT) control and Pdx1Cre/+; beta-cateninflox/flox beta-catenin null in the pancreas) embryonic pancreas to Affymetrix MOE430 2.0 GeneChip® arrays containing 45,101 well characterized mouse genes/ESTs.
Experiment types
transcription profiling by array, individual genetic characteristics
Contact
Citation
Wnt/beta-catenin signaling is required for development of the exocrine pancreas. James M Wells, Farzad Esni, Gregory P Boivin, Bruce J Aronow, William Stuart, Chelsea Combs, Angela Sklenka, Steven D Leach, Andrew M Lowy. BMC Dev Biol 7:4 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-7430.idf.txt
Sample and data relationshipE-GEOD-7430.sdrf.txt
Raw data (1)E-GEOD-7430.raw.1.zip
Processed data (1)E-GEOD-7430.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-7430.eSet.r
Links