E-GEOD-7379 - Transcription profiling of E.coli Fis knockout strain
Submitted on 27 March 2007, released on 15 June 2008, last updated on 27 March 2012
Fis is a nucleoid-associated protein in E. coli that is abundant during early logarithmic growth in rich medium but is in short supply during stationary phase. Its role as a transcriptional regulator has been demonstrated for an increasing number of genes. In order to gain insight into the global effects of Fis on E. coli gene expression during different stages of growth in rich medium, DNA microarray analyses were conducted in fis and wild type strains during early log, mid log, late log, and stationary growth phases. We used microarrays to detail the global impact of Fis on gene expression in Escherichia coli Experiment Overall Design: E.coli cells were were grown and samples were taken at different times during growth: 90 min (early logarithmic phase), 150 min (mid-logarithmic phase), 240 min (late logarithmic phase), and 360 min (early stationary phase) for RNA extraction and hybridization on Affymetrix microarrays. This was done in triplicate and the raw data was analyzed using Microarray Analysis Suite version 5.0 (Affymetrix).
transcription profiling by array, unknown experiment type
Effects of Fis on Escherichia coli gene expression during different growth stages. Meranda D Bradley, Michael B Beach, A P Jason de Koning, Timothy S Pratt, Robert Osuna. Microbiology (Reading, Engl) 153(Pt 9):2922-40 (2007)