E-GEOD-7364 - Transcription profiling of human neuroblastoma cell lines using a dene-resolution analysis method of DNA copy number variation using oligonucleotide expression microarrays

Status
Submitted on 26 March 2007, released on 15 June 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (4)
Description
We present a novel method of using commercial oligonucleotide expression microarrays for aCGH, enabling DNA copy number measurements and expression profiles to be combined using the same platform. This method yields aCGH data from genomic DNA without complexity reduction at a median resolution of approximately 17,500 base pairs. Due to the well-defined nature of oligonucleotide probes, DNA amplification and deletion can be defined at the level of individual genes and can easily be combined with gene expression data. Experiment Overall Design: Genomic DNA isolated from peripheral blood samples and from Neuroblastoma cell lines was fragmented by DNAseI and biotin labeled by terminal transferase. Labeled DNAs were hybridized to U133plus2.0 GeneChips (Affymetrix). Hybridization and other conditions were slightly modified from those suggested for 10K Mapping Arrays (Affymetrix) and washing conditions were carried out as suggested for 100K Mapping Arrays. Probe set signals were either generated using the RMA algorithm or using the in-house developed WPP algorithm. Copy-number variation between Neuroblastoma and normal DNA was calculated.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7364.idf.txt
Sample and data relationshipE-GEOD-7364.sdrf.txt
Raw data (1)E-GEOD-7364.raw.1.zip
Processed data (1)E-GEOD-7364.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-7364.eSet.r
Links