E-GEOD-7354 - Transcription profiling of C. elegans presenilin model

Status
Submitted on 23 March 2007, released on 15 June 2008, last updated on 10 June 2011
Organism
Caenorhabditis elegans
Samples (2)
Array (1)
Protocols (2)
Description
Regulation of presenilin genes. Presenilins are intramembrane aspartic proteases. These proteases are critical proteins in pathogenesis of Alzheimer's disease. The function of recently identified presenilin-homologous proteases (IMPAS or SPP)s is unknown. Our preliminary data in C.elegans model suggested the role of these proteins in early-development and , perhaps, in pathway related- to cholesterol -regulated signalling (Grigorenko et al, 2004, PNAS). The overall goal is to determine pattern of gene expression alterations in cells with knock-out or knock-down presenilin related proteins (IMPAS). The cultured cells, C.elegans and mouse models are planned to be used in this study. Here , we will determine gene expression patterns in C.elegans strain NL2099, fed with bacteria HT115, producing dsRNA for Ce-imp-2 gene (knock-down of imp-2 gene by RNAi )or GFP gene as a control. We hypothesize that novel family of presenilin-related proteases is important for cholesterol-regulated intracellular signalling and early development (including CNS/neuronal development and function). C.elegans (strain NL2099) on L1 stage were placed on agarose plates with bacteria HT115, producing dsRNA for: 1) Ce-imp-2 gene; 2) GFP as a control gene. After 48 hours at 20C , worms were washed in M9 buffer and total RNA was isolated by TRIzol method.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-7354.idf.txt
Sample and data relationshipE-GEOD-7354.sdrf.txt
Raw data (1)E-GEOD-7354.raw.1.zip
Processed data (1)E-GEOD-7354.processed.1.zip
Array designA-AFFY-60.adf.txt
R ExpressionSetE-GEOD-7354.eSet.r
Links