E-GEOD-7353 - Transcription profiling by array of Arabidopsis mutant for gid1a, gid1b and gid1c or for ga-1 after treatment with Gibberellin (GA3)

Status
Released on 15 June 2008, last updated on 30 April 2015
Organism
Arabidopsis thaliana
Samples (12)
Array (1)
Protocols (7)
Description
The phytohormone GA controls multiple important developmental processes in plants such as germination, elongation growth and flowering time. In this experiment, we look for early GA response genes in 7 day-old light-grown Arabidopsis seedlings. To this end we compare four data sets: (1) a GA biosynthesis mutant ga-1 (SALK_109115) mock treated for 1 hr; (2) a GA biosynthesis mutant ga-1 (SALK_109115) treated for 1 hr with 100 µM GA3; (3) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant mock treated for 1 hr; (4) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant treated for 1 hr with 100 µM GA3. In a comparison of the two ga-1 samples, GA regulated genes can be identified, and the assumption is that bona fide GA regulated genes are not responding in the gid1a-1 gid1b-1 gid1c-2 GA receptor mutant. Experiment Overall Design: We compare four samples of 7 day-old light-grown growth-medium grown seedlings (3 independent biological replicates each): (1) a GA biosynthesis mutant ga-1 (SALK_109115) mock treated for 1 hr; (2) a GA biosynthesis mutant ga-1 (SALK_109115) treated for 1 hr with 100 µM GA3; (3) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant mock treated for 1 hr; (4) a gid1a-1 gid1b-1 gid1c-2 GA receptor triple mutant treated for 1 hr with 100 µM GA3. In a comparison of the two ga-1 samples, GA regulated genes can be identified, and the assumption is that bona fide GA regulated genes are not responding in the gid1a-1 gid1b-1 gid1c-2 GA receptor mutant.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
The DELLA domain of GA INSENSITIVE mediates the interaction with the GA INSENSITIVE DWARF1A gibberellin receptor of Arabidopsis. Bjorn C Willige, Soumya Ghosh, Carola Nill, Melina Zourelidou, Esther M N Dohmann, Andreas Maier, Claus Schwechheimer.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7353.idf.txt
Sample and data relationshipE-GEOD-7353.sdrf.txt
Raw data (1)E-GEOD-7353.raw.1.zip
Processed data (1)E-GEOD-7353.processed.1.zip
Array designA-AFFY-2.adf.txt
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