E-GEOD-7149 - Transcription profiling of Anopheles gambiae alimentary canal

Status
Submitted on 27 February 2007, released on 6 November 2008, last updated on 27 March 2012
Organism
Anopheles gambiae
Samples (15)
Array (1)
Protocols (6)
Description
Although the basic anatomical sub-divisions of the larval mosquito gut were established several decades ago, information regarding their exact physiological roles is rather scarce. Several studies have reported differences between larval gut compartments in various morphological and physiological aspects. Unfortunately, the fragmentary and incomplete nature of this information makes it hard to establish clear links to the specific and/or unique physiological roles of each gut region. In this study, we attempted to identify transcripts significantly enriched in each gut section of the 4th instar Anopheles gambiae larva. We took advantage of a commercially-available microarray platform containing ~14,000 different An. gambiae transcripts (Affymetrix GeneChip® Plasmodium/Anopheles array, Affymetrix Inc., Santa Clara, CA). Based on the information obtained, we discuss the putative physiological roles of each major compartment of the larval gut, and identify potential new targets for larval control strategies. Experiment Overall Design: We attempted to identify transcripts significantly enriched in each gut section of the 4th instar Anopheles gambiae larva. Signal intensities of transcripts from each gut section were compared to those of a matching group of whole 4th instar larvae using t-tests. Transcripts showing an intensity value two-fold or higher (p < 0.05) in each gut section as compared to whole larvae were considered to be significantly enriched.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
A microarray-based analysis of transcriptional compartmentalization in the alimentary canal of Anopheles gambiae (Diptera: Culicidae) larvae. M Neira Oviedo, L Vanekeris, M D P Corena-McLeod, P J Linser. Insect Mol Biol 17(1):61-72 (2008)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-7149.idf.txt
Sample and data relationshipE-GEOD-7149.sdrf.txt
Raw data (1)E-GEOD-7149.raw.1.zip
Processed data (1)E-GEOD-7149.processed.1.zip
Array designA-AFFY-102.adf.txt
R ExpressionSetE-GEOD-7149.eSet.r
Links