E-GEOD-7101 - Transcription profiling of human p53+ and p53- fibroblasts treated with arsenite reveals exit from arsenite-induced mitotic arrest is p53 dependent

Status
Submitted on 21 February 2007, released on 11 November 2007, last updated on 2 May 2014
Organism
Homo sapiens
Samples (16)
Array (1)
Protocols (6)
Description
Microarray data from G2-synchronized p53(+) and p53(-) fibroblasts before and after 3 h release from cell cycle blockade in the presence of 5 uM sodium arsenite. Experiment Overall Design: Cells expressing p53 from a tet-off regulated construct were synchronized in G2 with a two-step procedure using 24 h aphidicolin treatment for initial G1 synchronization and 12 h of Hoechst 33342 to effect a G2 blockade. During Hoechst treatment, tetracycline was added to suppress p53 in half the cultures. Cells were then released into media containing 5 µM sodium arsenite and the appropriate concentration of tetracycline to maintaining p53 expression. mRNAs were collected at 0 h and 3 h after release from G2 synchrony.
Experiment types
transcription profiling by array, co-expression, compound treatment, in vitro
Contact
Xiaoqiang Xu
Citation
Exit from arsenite-induced mitotic arrest is p53 dependent. Samuel C McNeely, Xiaogiang Xu, B Frazier Taylor, Wolfgang Zacharias, Michael J McCabe, J Christopher States. Environ Health Perspect 114(9):1401-6 (2006)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-7101.idf.txt
Sample and data relationshipE-GEOD-7101.sdrf.txt
Raw data (1)E-GEOD-7101.raw.1.zip
Processed data (1)E-GEOD-7101.processed.1.zip
Array designA-AFFY-1.adf.txt
R ExpressionSetE-GEOD-7101.eSet.r
Links