E-GEOD-6872 - Transcription profiling by array of human sperm from normospermic and teratozoospermic individuals

Status
Released on 11 November 2007, last updated on 11 February 2014
Organism
Homo sapiens
Samples (21)
Array (1)
Protocols (8)
Description
Normal human spermatogenesis concludes with the formation of large numbers of morphologically well developed spermatozoa. While transcriptionally quiescent these cells carry an RNA payload that reflects the final spermiogenic phase of transcription. We report here the spermatozoal transcript profiles characteristic of normally fertile individuals and infertile males suffering from a consistent and severe teratozoospermia in which under 4% of spermatozoa are morphologically normal. RNA was extracted from the purified sperm cells of ejaculate and hybridized to Affymetrix U133 (v2) Microarrays. Spermatozoal RNAs were prepared from the semen samples of 21 individuals. An asymmetric dual block design was adopted with biological replicates in both blocks. 13 semen samples were assessed from normally fertile males who had fathered at least one child. 8 semen samples were assessed from infertile individuals with a severe and consistent heterogeneous teratozoospermia who showed no other abnormal semen parameters.
Experiment types
transcription profiling by array, co-expression, disease state
Contact
Citation
Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs. Adrian E Platts, David J Dix, Hector E Chemes, Kary E Thompson, Robert Goodrich, John C Rockett, Vanesa Y Rawe, Silvina Quintana, Michael P Diamond, Lillian F Strader, Stephen A Krawetz.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-6872.idf.txt
Sample and data relationshipE-GEOD-6872.sdrf.txt
Raw data (1)E-GEOD-6872.raw.1.zip
Processed data (1)E-GEOD-6872.processed.1.zip
Array designA-AFFY-44.adf.txt
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