E-GEOD-678 - Transcriptome of a mouse kidney cortical collecting duct cell line: effects of aldosterone and vasopressin
Submitted on 23 September 2003, released on 24 September 2003, last updated on 27 March 2012
In this study, we analyzed the transcriptome of a highly differentiated mouse clonal CCD principal cell line (mpkCCD(cl4)) and the changes in the transcriptome induced by aldosterone and vasopressin. Serial analysis of gene expression (SAGE) was performed on untreated cells and on cells treated with either aldosterone or vasopressin for 4 h. The transcriptomes in these three experimental conditions were determined by sequencing 169,721 transcript tags from the corresponding SAGE libraries. Limiting the analysis to tags that occurred twice or more in the data set, 14,654 different transcripts were identified, 3,642 of which do not match known mouse sequences. Statistical comparison (at P < 0.05 level) of the three SAGE libraries revealed 34 AITs (aldosterone-induced transcripts), 29 ARTs (aldosterone-repressed transcripts), 48 VITs (vasopressin-induced transcripts) and 11 VRTs (vasopressin-repressed transcripts). A selection of the differentially-expressed, hormone-specific transcripts (5 VITs, 2 AITs and 1 ART) has been validated in the mpkCCD(cl4) cell line either by Northern blot hybridization or reverse transcription-PCR. The hepatocyte nuclear transcription factor HNF-3-alpha (VIT39), the receptor activity modifying protein RAMP3 (VIT48), and the glucocorticoid-induced leucine zipper protein (GILZ) (AIT28) are candidate proteins playing a role in physiological responses of this cell line to vasopressin and aldosterone. Keywords: other
transcription profiling by SAGE
Jean-Marc Elalouf <firstname.lastname@example.org>, Alain Doucet, Alain Vandewalle, Bernard C Rossier, Dmitri Firsov, François Artiguenave, Jean-Daniel Horisberger, Marcelle Bens, Marie Nicod, Marjorie Flahaut, Maya Robert-Nicoud, Miguel Salinas, Patrick Wincker
Transcriptome of a mouse kidney cortical collecting duct cell line: effects of aldosterone and vasopressin. Robert-Nicoud M, Flahaut M, Elalouf JM, Nicod M, Salinas M, Bens M, Doucet A, Wincker P, Artiguenave F, Horisberger JD, Vandewalle A, Rossier BC, Firsov D.