E-GEOD-6674 - Transcription profiling of mouse AM-14 B cells stimulated through the B cell receptor (BCR) and/or Toll-like receptors (TLR)

Status
Submitted on 8 January 2007, released on 15 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (15)
Array (1)
Protocols (9)
Description
We have previously shown that rheumatoid factors (RF) produced by Fas-deficient autoimmune-prone mice typically bind autologous IgG2a with remarkably low affinity. Nevertheless, B cells representative of this RF population proliferate vigorously in response IgG2a/chromatin immune complexes through a mechanism dependent on the sequential engagement of the BCR and Toll-like receptor 9 (TLR9). To more precisely address the role of both receptors in this response, we analyzed the signaling pathways activated in AM14 B cells stimulated with these complexes. We found that the BCR not only serves to direct the chromatin complex to an internal compartment where it can engage TLR9 but also transmits a suboptimal signal that in combination with the signals emanating from TLR9 leads to NF?B activation and proliferation. Importantly, engagement of both receptors leads to the upregulation of a group of gene products, not induced by the BCR or TLR9 alone, that include IL-2. These data indicate that autoreactive B cells, stimulated by a combination of BCR and TLR9 ligands, acquire functional properties that may contribute to the activation of additional cells involved in the autoimmune disease process. Experiment Overall Design: 15 Samples, 3 replicates for each treatment
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Functional outcome of B cell activation by chromatin immune complex engagement of the B cell receptor and TLR9. Liliana Busconi, Jason W Bauer, Joseph R Tumang, Amy Laws, Kristin Perkins-Mesires, Abigail S Tabor, Christina Lau, Ronald B Corley, Thomas L Rothstein, Frances E Lund, Timothy W Behrens, Ann Marshak-Rothstein. J Immunol 179(11):7397-405 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-6674.idf.txt
Sample and data relationshipE-GEOD-6674.sdrf.txt
Raw data (1)E-GEOD-6674.raw.1.zip
Processed data (1)E-GEOD-6674.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-6674.eSet.r
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