E-GEOD-6561 - Transcription profiling of human embryonic stem cell line containining HSR H14, and parent H14 l

Status
Submitted on 18 December 2006, released on 14 June 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (6)
Description
Homogenous staining regions (hsr) are cytogenetic representations of a gene amplification. They are found exclusively in tumour cells. We found a hsr in the human embryonic stem cell (hESC) line H14, this is the first report of a hsr in hESCs. FISH and CGH studies showed that the hsr was derived from chromosome 17p11.2. The gene expression analysis studies were performed to identify the genes upregulated due to the hsr by comparison the the hsr-contianing H14 cells with a karyotypically normal parent H14 cell line. Experiment Overall Design: H14 HSR positive embryonic stem cells and H14 HSR negative cells were hybridised to Affymetrix U133 Plus 2.0 expression arrays. Replicates were also run using H14 positive and H14 negative cells from different passages.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Adaptation to culture of human embryonic stem cells and oncogenesis in vivo. Duncan E C Baker, Neil J Harrison, Edna Maltby, Kath Smith, Harry D Moore, Pamela J Shaw, Paul R Heath, Hazel Holden, Peter W Andrews. Nat Biotechnol 25(2):207-15 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-6561.idf.txt
Sample and data relationshipE-GEOD-6561.sdrf.txt
Raw data (1)E-GEOD-6561.raw.1.zip
Processed data (1)E-GEOD-6561.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-6561.eSet.r
Links