E-GEOD-6559 - Transcription profiling of primary human keratinocytes

Status
Submitted on 18 December 2006, released on 14 June 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (10)
Description
Oncogenic Ras induces epidermal cell growth arrest. Induction of the JNK/Ap1 signaling cascade by expression of MKK7 overcomes Ras-induced cell growth arrest in a manner dependent on AP1 fucntion. We used microarrays to detail the global programme of gene expression in response to MKK7, Ras activation and AP1 inhibition Experiment Overall Design: To study the gene expression profile responsive to Ras and MKK7 expression. We used retroviral expression system to express LacZ, MKK7, Ras, DNc-Jun, MKK7+Ras, and MKK7+Ras +DNc-Jun in primary human keratinocytes, and then extract total RNA from the transduced cells for gene expression analysis.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Tumor necrosis factor receptor 1/c-Jun-NH2-kinase signaling promotes human neoplasia. Jennifer Y Zhang, Amy E Adams, Todd W Ridky, Shiying Tao, Paul A Khavari. Cancer Res 67(8):3827-34 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-6559.idf.txt
Sample and data relationshipE-GEOD-6559.sdrf.txt
Raw data (1)E-GEOD-6559.raw.1.zip
Processed data (1)E-GEOD-6559.processed.1.zip
Array designA-AFFY-37.adf.txt
R ExpressionSetE-GEOD-6559.eSet.r
Links