E-GEOD-6556 - Transcription profiling by array of Arabidopsis heterozygous mutant for gh3.5 after inoculation with Pseudomonas syringae pv tomato DC3000(avrRpt2) against wild type counterparts and uninfected controls
Released on 14 June 2008, last updated on 6 August 2014
To reveal the underlying molecular mechanism of GH3.5 action in modulating the SA and auxin pathways, we performed transcriptional profiling of gh3.5-1D plants after infection with or without Pst DC3000(avrRpt2) on a global scale using the Affymetrix Arabidopsis ATH1 GeneChip Experiment Overall Design: Each 3 leaves of plants of 5-week-old wild type Columbia-0 and mutant gh3.5-1D (heterozygous) were inoculated with Pst DC3000(avrRpt2)at 105 cfu mL-1. Leaves were harvested at 0 h (uninoculated) and 48 hours after inoculation. Three biological repeats were performed on Columbia-0 (wild-type, Col-0) and gh3.5-1D (mutant) after infection with or without Pst DC3000(avrRpt2), respectively.
transcription profiling by array
Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-Pseudomonas syringae interaction. Zhongqin Zhang, Qun Li, Zhimiao Li, Paul E Staswick, Muyang Wang, Ying Zhu, Zuhua He. , Europe PMC 17704230