E-GEOD-587 - Transcription profiling of murine neurosphere cultures carrying a Bcl2 transgene and treated with histone deacetylase inhibitor Trichostatin A and demethylating agent AzaC to study DNA methylation and histone deacetylation in neural stem cells

Status
Submitted on 12 August 2003, released on 19 December 2007, last updated on 27 March 2012
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (1)
Description
Neurosphere cultures were established from individually isolated E 14.5 forebrains of mouse embryos that carry a bcl2 transgene. Single-cell suspensions were prepared, seeded at 1x105 cells/ml and treated for two days with 150 nM Trichostatin A (TSA; histone deacetylase inhibitor), 500 nM 5-Aza-2-deoxycytidine (AzaC; demethylating agent) or both compounds, or left untreated. Two independent experiments were performed.
Experiment types
transcription profiling by array, co-expression, compound treatment
Contact
Citation
Pluripotency associated genes are reactivated by chromatin-modifying agents in neurosphere cells. Ruau D, Ensenat-Waser R, Dinger TC, Vallabhapurapu DS, Rolletschek A, Hacker C, Hieronymus T, Wobus AM, Müller AM, Zenke M. Stem Cells 26(4):920-6 (2008)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-587.idf.txt
Sample and data relationshipE-GEOD-587.sdrf.txt
Processed data (1)E-GEOD-587.processed.1.zip
Array designA-AFFY-6.adf.txt
Links