E-GEOD-58359 - Gene expression changes after loss of Hoxa9 in transformed HSCs [HOXA9 RNA-seq]

Status
Released on 11 June 2014, last updated on 14 August 2014
Organism
Mus musculus
Samples (2)
Protocols (4)
Description
Characterization of gene expression changes 72 hours after withdrawal of tamoxifen in murine hematopoietic progenitors transformed by Hoxa9-ER/Meis1 using RNAseq. In the presence of tamoxifen (4OHT), Hoxa9-ER localizes to the nucleus of cells allowing for transformation, while withdrawal of 4OHT (culture in EtOH) leads to loss of nuclear Hoxa9-ER. Loss of Hoxa9-ER leads to a decrease in cellular proliferation and differentiation along the myeloid lineage. Examination of gene expression by RNAseq in two conditions in biological replicates.
Experiment type
RNA-seq of coding RNA 
Contacts
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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