E-GEOD-58176 - Transcriptional profiling of E. coli cultures exposed to tellurite 0.5 µg/ml

Status
Released on 30 July 2014, last updated on 2 August 2014
Organism
Escherichia coli K-12
Samples (4)
Array (1)
Protocols (7)
Description
E. coli cultures were exposed to tellurite 0.5 µg/ml during 15 min. Total RNA was extracted and cDNA labeled probes were generated by reverse transcription using Alexa 555 and Alexa 647 fluorophores. These probes were used to hybridize genomic slides containing genomic arrays to determine global transcriptional changes. Two-conditions experiment, antibacterial vs. Untreated control cells. Biological replicates: 2 control, 2 toxicants exposed cells, independently grown and harvested. One replicate per array. Dye swap conditions.
Experiment type
transcription profiling by array 
Contacts
David Enrique Loyola <dloyolac@gmail.com>, David E Loyola, Roberto C Molina-Quiroz
Citation
Global transcriptomic analysis uncovers a switch to anaerobic metabolism in tellurite-exposed Escherichia coli. Molina-Quiroz RC, Loyola DE, D�az-V�squez WA, Arenas FA, Urz�a U, P�rez-Donoso JM, V�squez CC. , Europe PMC 25049169
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-58176.idf.txt
Sample and data relationshipE-GEOD-58176.sdrf.txt
Raw data (1)E-GEOD-58176.raw.1.zip
Processed data (1)E-GEOD-58176.processed.1.zip
Array designA-GEOD-15818.adf.txt
Links