E-GEOD-57347 - Regulation of p21 by TWIST2 contributes to its tumor suppressor function in human acute myeloid leukemia

Status
Released on 7 May 2014, last updated on 3 June 2014
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (5)
Description
TWIST2 is deregulated in about 1/3 human acute myeloid leukemia (AML) patients partially due to hypermethylation. Overexpression of TWIST2 in various AML cell lines and primary AML cells leads to growth arrest, reduced colony forming cell (CFC) capacity, inhibited cell migration and tumorigenic ability in nude mice, suggesting its tumor suppressor role in AML. To explore the molecular insight of how TWIST2 acts as a tumor suppressor in AML cells, control and TWIST2 expressed THP-1 cells have been analyzed with microarray. 4 independent biological replicates of control and TWIST2 expressed THP-1 cells have been used for RNA extraction and microarray analysis with Agilent platform.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-57347.idf.txt
Sample and data relationshipE-GEOD-57347.sdrf.txt
Raw data (1)E-GEOD-57347.raw.1.zip
Array designA-AGIL-28.adf.txt
Links