E-GEOD-5730 - Transcription profiling by array of Arabidopsis embryo tissues

Status
Released on 14 June 2008, last updated on 17 January 2012
Organism
Arabidopsis thaliana
Samples (27)
Array (1)
Protocols (2)
Description
The aim of this BBSRC-funded project is to develop laser-capture microdissection (LCMD) to isolate small cell clusters in different regions of arabidopsis embryos at different stages of development; to develop RNA amplification procedures on dissected tissue sampes; and to use DNA microarray techniques to investigate global transcriptional differences between samples. Cryosectioned embryos of ecotype Col-O of globular, heart and torpedo stage were used to isolate cell clusters from the apical and basal regions, for RNA isolation and amplification. !Samples will be provided as T7-primed cDNA, with three biological replicates for each tissue to be analysed. Each replicate comprises cDNA from pooled tissue samples from ca. 15 embryos. The experimental details have been discussed with Sean May et al. at NASC. Experimenter name = Stuart Casson and Matthew Spencer; Experimenter phone = 0191 374 7356; Experimenter fax = 0191 374 2417; Experimenter institute = Durham University; Experimenter address = Integrative Cell Biology Laboratory; Experimenter address = School of Biological Sciences; Experimenter address = Durham University; Experimenter address = South Road; Experimenter address = Durham; Experimenter zip/postal_code = DH1 3LE; Experimenter country = UK Experiment Overall Design: 27 samples were used in this experiment
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5730.idf.txt
Sample and data relationshipE-GEOD-5730.sdrf.txt
Raw data (1)E-GEOD-5730.raw.1.zip
Processed data (1)E-GEOD-5730.processed.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-5730.eSet.r
Links