E-GEOD-57281 - RNA helicases DDX5 and DDX17 dynamically orchestrate transcription, microRNA and splicing programs in cell differentiation

Status
Released on 6 May 2014, last updated on 12 May 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (7)
Description
RNA helicases DDX5 and DDX17 are members of a large family of highly conserved proteins involved in gene expression regulation, although their in vivo targets and activities in biological processes like cell differentiation, that requires reprogramming of gene expression programs at multiple levels, are not well characterized. In this report, we uncovered a new mechanism by which DDX5 and DDX17 cooperate with hnRNP H/F splicing factors to define epithelial- and myoblast-specific splicing subprograms. We next observed that downregulation of DDX5 and DDX17 protein expression during epithelial to mesenchymal transdifferentiation and during myogenesis contributes to switching splicing programs during these processes. Remarkably, this downregulation is mediated by the production of microRNAs induced upon differentiation in a DDX5/DDX17-dependent manner. Since DDX5 and DDX17 also function as coregulators of master transcriptional regulators of differentiation, we propose to name these proteins “master orchestrators” of differentiation, that dynamically orchestrate several layers of gene expression. 6 samples of MCF7 cells exposed to different treatments were analyzed: 3 x siCTRL ; 3 x si(DDX5-17) AND 6 samples of MCF10 cells exposed to different treatments were analyzed: 3 x siCTRL ; 3 x si(DDX5-17)
Experiment type
transcription profiling by array 
Contacts
Didier Auboeuf <geo@ncbi.nlm.nih.gov>, Cyril F Bourgeois, Eleonora Zonta, Etienne Dardenne, Fatima Z Chakrama, François-Olivier Desmet, Helen Neil, Hussein Mortada, Laurent Fattet, Lise Gratadou, Léon-Charles Tranchevent, Marie-Pierre Lambert, Martin Dutertre, Mathieu Deygas, Micaela Polay Espinoza, Ruth Rimokh, Samaan Samaan, Sophie Germann
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-57281.idf.txt
Sample and data relationshipE-GEOD-57281.sdrf.txt
Raw data (2)E-GEOD-57281.raw.1.zip, E-GEOD-57281.raw.2.zip
Processed data (1)E-GEOD-57281.processed.1.zip
Array designA-AFFY-143.adf.txt
Links