E-GEOD-56936 - Expression data from NEAT1 regulated genes with or without polyI:C
Released on 7 May 2014, last updated on 3 June 2014
Although thousands of long non-coding RNAs (lncRNAs) are localized in the nucleus, only a few dozen have been functionally characterized. We found that nuclear paraspeckle assembly transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, is induced by poly I:C stimulation, resulting in excess formation of paraspeckles. Using microarray analysis, we investigated whether NEAT1 induction followed by excess formation of paraspeckles was involved in poly I:C-inducible gene expression. We want to know the NEAT1-regulated genes. To the end, HeLa TO cells with and without poly I:C stimulation and NEAT1-knock down cells with and without poly I:C stimulation and cells transfected with mock plasmid or Neat1v2 expression plasmid alone were used for RNA extraction and hybridization on Affymetrix microarrays.
transcription profiling by array
Akihisa Kato, Atsushi Kawaguchi, Chieko Kai, Gen Akizuki, Hiroki Sato, Katsutoshi Imamura, Kazuhisa Sekimizu, Kiyomi Kaneki, Kyosuke Nagata, Michiko Kumakura, Mika Kobayashi, Misako Yoneda, Naoto Imamachi, Nobuyoshi Akimitsu, Takahide Kohro, Tatsuhiko Kodama, Tetsushi Yada, Tsuyoshi Inoue, Yasushi Kawaguchi, Youichiro Wada