E-GEOD-56112 - Expression Array analysis of MTAP in HT080 cells

Status
Released on 30 April 2014, last updated on 3 June 2014
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (6)
Description
Methylthioadenosine Phosphorylase (MTAP) is a tumor suppressor gene that encodes an enzyme responsible for the catabolism of the polyamine byproduct 5′deoxy-5′-methylthioadenosine (MTA). To elucidate the mechanism by which MTAP inhibits tumor formation, we have created isogenic MTAP+ and MTAP- HT1080 fibrosarcoma cells. In this experiment we have performed expression array analysis on MTAP-, MTAP+, and MTAP+ cells treated with the MTAP inhibitor MT-DADMe-ImmA. Three biological replicates of each sample were grown and analyzed. M- is MTAP-. M+ is MTAP+, and M+I is MTAP treated with inhibitor (48 hours).
Experiment type
transcription profiling by array 
Contacts
warren kruger <warren.kruger@fccc.edu>, Baiqing Tan, Michael Slifker, Warren Kruger, Yue-Sheng Li
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-56112.idf.txt
Sample and data relationshipE-GEOD-56112.sdrf.txt
Raw data (1)E-GEOD-56112.raw.1.zip
Processed data (1)E-GEOD-56112.processed.1.zip
Array designA-AFFY-44.adf.txt
Links