E-GEOD-56025 - The impact of calorie restriction and diet-induced obesity on microRNA expression

Status
Released on 30 April 2014, last updated on 3 June 2014
Organism
Mus musculus
Samples (32)
Array (1)
Protocols (6)
Description
We analyzed the impact of calorie restriction and diet-induced obesity on expression of microRNAs in the mouse colon. For this analysis, data was LOESS normalized in R. Data was then imported into BRB Array for analysis. We identified microRNAs that were altered in response to calorie-restriction and diet-induced obesity Total mRNA was extracted from mouse colon tissue that was flash frozen immediately after euthaniasia. A total of 6 colons per the three groups were used for microarray analysis. Briefly, 5 ug of RNA were biotin labeled and hybridized to OSU-CCC microRNA microarrays version 4.0. We then analyzed differences in expression with BRB Array.
Experiment type
transcription profiling by array 
Contacts
Susan Elizabeth Olivo-Marston <geo@ncbi.nlm.nih.gov>, Aaron J Schetter, Curtis C Harris, Mohammed Khan, Susan E Olivo-Marston
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-56025.idf.txt
Sample and data relationshipE-GEOD-56025.sdrf.txt
Raw data (1)E-GEOD-56025.raw.1.zip
Processed data (1)E-GEOD-56025.processed.1.zip
Array designA-GEOD-13716.adf.txt
Links