E-GEOD-55851 - Whole genome gene expression profiling in CD4+ T-cells, FACS-sorted based on CADM1/CD7 expression levels, in Adult T-cell Leukemia-lymphoma (ATL) patients, HTLV-1 carriers, and in healthy volunteers

Status
Released on 2 June 2014, last updated on 7 June 2014
Organism
Homo sapiens
Samples (21)
Array (1)
Protocols (7)
Description
CADM1 overexpresion and stepwise downregulation of CD7 in CD4+T-cells from ATL patients and HTLV-1 carriers accurately reflect the process of disease, i.e., oligoclonally and clonally exanding cells are accumulated in D (CADM1posCD7dim) and N (CADM1posCD7neg) populations, respectively. Moreover, gene expression profiling of each subpopulation supported that notion. PBMCs of asymptomatic carriers (ACs) and ATLs were subgrouped based on CADM1 and CD7 expression levels by multicolor flow cytometry. FACS-sorted subpopulations were then subjected to extraction of total RNA, followed by Cy-3 labeling and human whole genome gene expression microarray analyses.
Experiment type
transcription profiling by array 
Contacts
Kaoru Uchimaru, Kazumi Nakano, Seiichiro Kobayashi, Toshiki Watanabe
Citation
CADM1 Expression and Stepwise Downregulation of CD7 Are Closely Associated with Clonal Expansion of HTLV-I-Infected Cells in Adult T-cell Leukemia/Lymphoma. Kobayashi S, Nakano K, Watanabe E, Ishigaki T, Ohno N, Yuji K, Oyaizu N, Asanuma S, Yamagishi M, Yamochi T, Watanabe N, Tojo A, Watanabe T, Uchimaru K. , Europe PMC 24727323
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55851.idf.txt
Sample and data relationshipE-GEOD-55851.sdrf.txt
Raw data (1)E-GEOD-55851.raw.1.zip
Processed data (1)E-GEOD-55851.processed.1.zip
Array designA-GEOD-10332.adf.txt
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