E-GEOD-55647 - Gene expression analysis of Mycobacterium tuberculosis high-persister (hip) mutants.

Status
Released on 7 April 2014, last updated on 8 April 2014
Organism
Mycobacterium tuberculosis
Samples (26)
Array (1)
Protocols (7)
Description
Bacterial persister cells are phenotypic variants of regular cells that are tolerant to antibiotics. High-persister (hip) mutants of Mycobacterium tuberculosis produce 10- to 1,000-fold more persister cells than the wild type strain when challenged with various antibiotics. Comparison of gene expression pattern of the hip mutants may provide clues as to the genetic mechanisms underlying persister formation. Transcriptome analysis will provide information on what differentiates M. tuberculosis hip strains from regular strains, which will be useful in the development of anti-persister therapy for persister cell eradication. Twelve independent M. tuberculosis hip mutants and the wild type strain were grown to stationary phase in duplicate. To avoid analyzing the drug effect, total RNA was extracted from the cultures prior to the addition of antibiotic for hybridization to Affymetrix microarrays. Data analysis was performed by comparing the hip mutants to the wild type control.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-55647.idf.txt
Sample and data relationshipE-GEOD-55647.sdrf.txt
Raw data (1)E-GEOD-55647.raw.1.zip
Processed data (1)E-GEOD-55647.processed.1.zip
Array designA-GEOD-17082.adf.txt
Links