E-GEOD-554 - Transcription profiling of mouse dorsal aorta derived mesangioblast clone comparison- U74B
Submitted on 23 July 2003, released on 13 June 2008, last updated on 27 March 2012
Mouse dorsal aorta from E9.5 mouse embryos (C57BL6) was grown as an explant culture as described elsewhere (De Angelis et al., 1999). After 5 days the explant was dissociated into a single cell suspension, and plated at limiting dilution on a feeder layer of Mitomycin C-treated primary embryonic mouse fibroblasts, in 96 multiwell plates in complete medium, RPMI 20% Fetal Calf Serum. After 1 week, mesangioblast clones appeared approximately 2-4% of the wells. When the clones had grown to approximately 103 cells they were passed on gelatin-coated dishes, and further expanded. Clones D16 and D351 were used for microarray analysis.
transcription profiling by array, cell type comparison
Msx2 and necdin combined activities are required for smooth muscle differentiation in mesoangioblast stem cells. Silvia Brunelli, Enrico Tagliafico, Fernanda G De Angelis, Rossana Tonlorenzi, Silvia Baesso, Sergio Ferrari, Michio Niinobe, Kazuaki Yoshikawa, Robert J Schwartz, Irene Bozzoni, Stefano Ferrari, Giulio Cossu. Circ Res 94(12):1571-8 (2004)