E-GEOD-55061 - Expression data from RCAS PDGFB/Nestin tv-A glioma cells within Ptprd+/+p16-/- (n=2), Ptprd+/-p16-/- (n=2), and Ptrpd-/-p16-/- (n=3) mice

Status
Released on 2 June 2014, last updated on 7 June 2014
Organism
Mus musculus
Samples (7)
Array (1)
Protocols (7)
Description
PTPRD is a tumor suppressor of glioma that is frequently co-deleted with CDKN2A/p16. We show that Ptprd and p16 cooperate to promote gliomagenesis in the RCAS PDGFB / Nestin tv-A glioma mouse model. We found unique gene expression changes within tumor cells of Ptprd+/-p16-/- vs. Ptprd+/+p16-/- and Ptprd-/-p16-/- tumor cells. Neonatal mice were injected with RCAS PDGFB GFP. At symptoms, or at 12 weeks post injection, GFP+DAPI- tumor cells were sorted for RNA extraction and hybridization on Affymetrix microarrays. The mouse strain used was a mixed background of C57/BL6 and FVB/N. Ptprd mice were from Uetani et al. 2000 and p16/Nestin-tvA mice were from Tchouganouva et al. 2007.
Experiment type
transcription profiling by array 
Contacts
Timothy Chan <chant@mskcc.org>, Berenice Ortiz, Timothy A Chan
Citation
Loss of the tyrosine phosphatase PTPRD leads to aberrant STAT3 activation and promotes gliomagenesis. Ortiz B, Fabius AW, Wu WH, Pedraza A, Brennan CW, Schultz N, Pitter KL, Bromberg JF, Huse JT, Holland EC, Chan TA. , Europe PMC 24843164
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-55061.idf.txt
Sample and data relationshipE-GEOD-55061.sdrf.txt
Raw data (1)E-GEOD-55061.raw.1.zip
Processed data (1)E-GEOD-55061.processed.1.zip
Array designA-AFFY-36.adf.txt
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