E-GEOD-5465 - Transcription profiling by array of Arabidopsis overexpressing rice topoisomerase 6 genes

Status
Released on 13 June 2008, last updated on 25 January 2012
Organism
Arabidopsis thaliana
Samples (6)
Array (1)
Protocols (4)
Description
10-day-old wild-type and homozygous transgenic Arabidopsis seedlings (overexpressing OsTOP6A3 and OsTOP6B) grown under normal growth conditions were used for total RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Experiment Overall Design: The experiment involves the study of differential gene expression in transgenic Arabidopsis seedlings overexpressing rice topoisomerase 6 subunit A (OsTOP6A3) and subunit B (OsTOP6B) cDNAs as compared to wild-type.Two biological replicates for each sample were used for microarray analysis.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-5465.idf.txt
Sample and data relationshipE-GEOD-5465.sdrf.txt
Raw data (1)E-GEOD-5465.raw.1.zip
Processed data (1)E-GEOD-5465.processed.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-5465.eSet.r
Links