E-GEOD-5431 - Transcription profiling of Arabidopsis with post-transcriptionally silenced Myosin XI-K
Submitted on 1 August 2006, released on 13 June 2008, last updated on 10 June 2011
This study was aimed to determine roles of individual myosins in organelle trafficking in plant cell. Expression of Myosin XI-K was suppressed in transgenic A.thaliana by using RNAi approach. To ensure that the RNAi-induced gene silencing was specific and did not affect expression of other myosins, a microarray analysis was performed. Experiment Overall Design: Total RNA was purified from A.thaliana plants transformed with an inverted repeat construct harbouring a sequence derived from Myosin XI-K ORF. 3 independent groups of plants were used for comparison of the expression profiles of 17 myosin RNAs to a control group comprised of plants constitutively expressing GUS ORF as a transgene.
transcription profiling by array, cellular modification