E-GEOD-5370 - Transcription profiling of human muscle biopsies from pre-treatment dermatomyostitis patients vs. normal controls to define molecular pathways in muscle of myositis patients.
Submitted on 21 July 2006, released on 26 October 2007, last updated on 10 June 2011
Idiopathic inflammatory myopathies (polymyositis and dermatomyositis) are heterogeneous group of muscle disorders of unknown etiology.The pathogenic pathways responsible for muscle fiber damage and dysfunction in myositis are not currently well defined. Identification of such pathways may help to design novel therapeutic interventions and also help to develop diagnostic tests. Experiment Overall Design: Muscle biopsies from a separate group of 5 adult untreated female DM patients were profiled and compared to muscle tissue of normal human healthy volunteers to define molecular pathways in muscle of myositis patients. Confirm and map key pathway members to specific cell types in the muscle tissue of patients and controls using RT-PCR, Western blotting and Immunolocalization.
transcription profiling by array, co-expression, disease state