E-GEOD-5355 - Transcription profiling of mouse small intestine intraepithelial lymphocytes (IEL) vs. TCR ab+ CD8b+ and TCR gd+ cells

Status
Submitted on 19 July 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (3)
Description
Mouse small intestine intraepithelial lymphocytes (IEL) that express a ab TCR and CD8aa homodimers are an enigmatic T cell subset, as their specificity and in vivo function remain to be defined. To gain insight into the nature of these cells, we performed global gene expression profiling using microarray analysis, combined with PCR and flow cytometry to determine the level of expression of selected genes. Using these methods, TCR ab+ CD8aa IEL were compared to their TCR ab+ CD8b+ and TCR gd+ counterparts. Experiment Overall Design: In this study, the three IEL populations were isolated by cell sorting from a pool of IELs from 20 mice. RNA from each IEL subset was used to probe one microarray. A replicate experiment was performed on a seperate occasion using a new pool of 20 mice and three additional microarrays were used.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Steve W. Granger
Citation
Mouse TCRalphabeta+CD8alphaalpha intraepithelial lymphocytes express genes that down-regulate their antigen reactivity and suppress immune responses. Timothy L Denning, Steve W Granger, Steve Granger, Daniel Mucida, Ryan Graddy, Georges Leclercq, Weiguo Zhang, Karen Honey, Jeffrey P Rasmussen, Hilde Cheroutre, Alexander Y Rudensky, Mitchell Kronenberg. J Immunol 178(7):4230-9 (2007)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-5355.idf.txt
Sample and data relationshipE-GEOD-5355.sdrf.txt
Processed data (1)E-GEOD-5355.processed.1.zip
Array designA-AFFY-6.adf.txt
Links