E-GEOD-5333 - Transcription profiling of mouse epididymis throughout development to identify cell-specific genes that can be used as biomarkers for key differentiation events
Submitted on 18 July 2006, released on 13 November 2007, last updated on 10 June 2011
The overall objective of this proposal is to map the temporal and spatial dynamics of gene expression in the fetal mouse testis at key developmental timepoints. Urogenital tract malformations are the most common birth defects in males and their incidence together with other male reproductive health concerns such as reduced fertility and testicular cancer are reportedly on the rise in the human population. To better understand the impact of genetic factors and environmental influences on testicular development, it is important to first understand normal gene expression patterns and signaling cascades within the fetal testis during development. The goal of this study is to identify cell-specific genes that can be used as biomarkers for key differentiation events. Experiment Overall Design: Day to day comparison in whole epididymus throughout development with triplicates at each time point. Each sample is a pair of epididymus from a single animal and each animal is taken from a different dam.
transcription profiling by array, co-expression, development or differentiation