E-GEOD-5321 - Transcription profiling by array of Drosophila mutant for otd-uvi
Released on 13 June 2008, last updated on 7 November 2011
Comparative global gene expression analysis was caried out using pupal head tissue of otd-uvi an eye specific mutant allele of Otd and wild type control Canton S., in order to delineate the function of homeobox transcription factor Otd in Drosophila photoreceptor development and function. Experiment Overall Design: The Drosophila strain Canton S was used as wild type for comparative gene expression analysis with the mutant strain otd-uvi. For each biological replicate, samples were derived from independent RNA extractions consisting of 15-20 pupal heads. Pupae were dissected at 80 hr APF and immediately transferred to 600ul of trizol (GIBCO/BRL). RNA extraction was performed according to manufacturers instructions. Qualitative and quantitative analysis of the total RNA was carried out by capillary electrophoresis on a RNA6000 Nano Assay chip, with Agilent 2100 Bioanalyzer (Agilent Technologies USA) Biotin labeled probes were generated from 10ug total RNA according to Affymetrix protocol.
transcription profiling by array, unknown experiment type
Analysis of the Otd-dependent transcriptome supports the evolutionary conservation of CRX/OTX/OTD functions in flies and vertebrates. Ranade SS, Yang-Zhou D, Kong SW, McDonald EC, Cook TA, Pignoni F. , Europe PMC 18241855