E-GEOD-5320 - Transcription profiling of brains from wild type and beta4 nicotinic acetylcholine receptor (nAChR) subunit deficient mice to identify genes that might be related to beta4-containing nAChRs activity
Submitted on 16 July 2006, released on 27 November 2007, last updated on 27 March 2012
Studies using mice with beta4 nicotinic acetylcholine receptor (nAChR) subunit deficiency (beta4-/- mice) helped reveal the roles of this subunit in bradycardiac response to vagal stimulation, nicotine-induced seizure activity and anxiety. In order to identify genes that might be related to beta4-containing nAChRs activity, we compared the mRNA expression profiles of brains from beta4-/- and wild-type mice using Affymetrix U74Avr2 microarray. Experiment Overall Design: The experimental groups included six wild-type mice and five beta4-/- mice. All mice were 6-9 weeks old. Total RNA was extracted from whole brain tissue of the mice.
transcription profiling by array, co-expression, individual genetic characteristics
Differential brain transcriptome of beta4 nAChR subunit-deficient mice: is it the effect of the null mutation or the background strain?. Merav Kedmi, Avi Orr-Urtreger. Physiol Genomics 28(2):213-22 (2007)