E-GEOD-52859 - Differential gene expression in ap2-g and ap2-g2 mutants of Plasmodium berghei

Status
Released on 3 December 2013, last updated on 3 June 2014
Organism
Plasmodium berghei
Samples (16)
Array (1)
Protocols (6)
Description
Commitment to and completion of sexual development are essential for malaria parasites to be transmitted through mosquitoes. The molecular mechanism(s) responsible for these processes however, remain largely unknown. We have identified two transcription factors (both belonging to the AP2 family) essential for gametocytogenesis. AP2-G mutants are characterised by a complete inability to produce gametocytes. In AP2-G2 mutants the gametocytaemia is very significantly reduced but not completely abolished. We have performed the microarray experiments in order to cokmpare the transcriptomes of these mutantnts to the WT parasites and between each other. As P.berghei parasites are characterised by asynchronous development in the rodent host, the different stage composition of the sample would impact the analysis. Therefore parasites were harvested and matured in in vitro to the schizont stage Pairwaise comparison between the mutants and parental line was performed. 3 biological replicates of each condition were used.
Experiment type
transcription profiling by array 
Contacts
Oliver Billker <ob4@sanger.ac.uk>, K K Modrzynska, O Billker
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-52859.idf.txt
Sample and data relationshipE-GEOD-52859.sdrf.txt
Raw data (1)E-GEOD-52859.raw.1.zip
Processed data (1)E-GEOD-52859.processed.1.zip
Array designA-GEOD-18005.adf.txt
Links