9 protocols
AccessionNameType
P-GSE52522-3
normalization data transformation protocol
The raw signal was quantile-normalized across samples in R. ID_REF = VALUE = quantile-normalized signal idelta-3.Detection Pval =
P-GSE52522-9
array scanning protocol
Scan protocol for Illumina MouseRef-8 v2.0 expression beadchip
P-GSE52522-2
normalization data transformation protocol
The raw signal was quantile-normalized across samples in R. ID_REF = VALUE = quantile-normalized signal idelta-2.Detection Pval =
P-GSE52522-1
normalization data transformation protocol
The raw signal was quantile-normalized across samples in R. ID_REF = VALUE = quantile-normalized signal idelta-1.Detection Pval =
P-GSE52522-8
hybridization protocol
Hyb protocol for Illumina MouseRef-8 v2.0 expression beadchip
P-GSE52522-7
labelling protocol
label protocol for Illumina MouseRef-8 v2.0 expression beadchip
P-GSE52522-4
sample treatment protocol
Pancreata were perfused through the common bile duct. After removing endogenous islets, the acinar fraction was digested with liberase and Elastase. Roche) into single cells.
P-GSE52522-5
growth protocol
Rag1-/- were obtained from Jackson Labs. For delta- cell induction, 100ul (>1x 109 pfu) of purified adenovirus was directly injected into the splenic lobe of the dorsal pancreas.
P-GSE52522-6
nucleic acid extraction protocol
Cherry+ induced beta cells were isolated by fluorescent activated cell sorting (FACS) with FACSaria (BD Bioscience), RNA samples were extracted by Trizol