normalization data transformation protocol
CEL files were processed using Affymetrix GCOS software and normalized with RMA method. RMA files were imported into GeneSpring 7.3 software (Agilent Technologies) and further analyzed. ID_REF = VALUE = RMA signal intensity
array scanning protocol
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Following fragmentation, fragmented cDNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Gene 1.0 ST Array. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (GeneChip® WT Terminal Labeling and Controls Kit P/N 901525 1, Affymetrix).
nucleic acid extraction protocol
Total RNA was extracted using an RNeasy Mini Kit (Qiagen).
Cells were grown in RPMI 1640 with 10% FBS, 5% penicillin/streptomycin
sample treatment protocol
No Chemicalls were treated